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Immunofluorescence microscopy for p50 and GADD45A was performed as previously described [ 76].
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Phase separation induced by a two-step temperature jump was studied by time-resolved light scattering and scanning electron microscopy for polystyrene/poly 2-chlorostyrene) blends.
Cytotoxicity was evaluated by fluorescent time-lapse microscopy for 48 h using the MetaMorph software (Molecular Devices).
For each sample, the number of cells per droplet was measured by microscopy for 100 different droplets (Figure 3A).
We used high-speed spinning disc confocal microscopy for 3D single-granule tracking in the cytosol and TIRF microscopy for 2D single-granule tracking at the PM to investigate LG movement.
Finally, NIH3T3 cells were followed in video time-lapse microscopy for 24 h and migration was quantitatively evaluated by recording the paths followed by each cell.
We imaged the RMS (Fig. 6E) with two photon microscopy for 5 hours: 2 hours (pre-treatment) followed by perfusion with either 10 ng/ml TGF-α or aCSF (control) for 3 hours (Fig. 6G; Movie S6).
To establish whether endogenous Dsg3 was expressed in a similar distribution pattern we carried out fluorescence confocal microscopy for Dsg3 and Dp in untransfected HaCaT keratinocytes which express high levels of Dsg3 (Fig 1D).
To examine the migration velocity and direction of transplanted cells in different hosts, we took time-lapse movies under epifluorescent microscopy for 6 embryos in each treatment and monitored about 8 10 cells in each embryo.
To precisely monitor the status of hPaf1 expression during mitosis, we compared ten different fields of confocal microscopy for Panc1 cells in T+12 (double thymidine block followed by 12 hours of release) and N0 (thymidine/nocodazole block) that synchronized the cells in late G2/early mitosis (checked by flow cytometry).
Imaging was conducted via confocal microscopy for LAMP2 and EEA1.
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