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Certain techniques are also hot property at the moment: microarrays, RNAi, and groovy confocal microscopy, for example.
The characterization of these often complex and on the nanoscale structured composite materials is usually done by vacuum based methods such as nitrogen physisorption or scanning electron microscopy, for example.
In STED microscopy, for example, a second laser is used to transfer the fluorophore from the excited state to the ground state without fluorescing.
When using fluorescent dyes for multiphoton microscopy, for example, the lifetime of the dye is quite short (in the nanosecond range).
This flexibility indicates that the synthesis process could be adapted to mimic other types of cellular material commonly analyzed using bright-field microscopy, for example, lung or oral cavity smears.
The morphology of Opticell-grown yeast in spaceflight was indistinguishable from static controls when observed by light microscopy; for example, budding pattern, overall shape, and size were not detectably different in the two conditions.
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Electron microscopy can, for example, identify the absence of α-granules in gray platelet syndrome.
These features are known to reflect the reorganization of actin microfilaments in viable migrating or invading cells, as demonstrated by previous fluorescence microscopy studies (for example, [ 40]).
Pattern interpretation of ANCA is characterized by human bias and high variability due to methodological issues such as differing fixation protocols for neutrophils and fluorescence microscopy components (for example, lamps, filters, objectives) [ 11].
Microscopy based techniques, for example spectral karyotyping and fluorescent in situ hybridization, yield a much more visual image of chromosome fragments and their instability.
This can be realised using intravital microscopy, as is, for example, undertaken in neurological studies in live rodents,[ 49] or by using endoscopy.
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