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To measure fluorescence signals in nuclei and cytoplasm, at least 50 cells were scanned by confocal microscopy for each of the different conditions.
Because of the variability in survival of wild-type spores in different flyspecks, multiple flyspecks were examined by DIC microscopy for each mutant and survival was quantified by counting the ratio of intact to ghost spores.
One biopsy was obtained for assessment by microscopy for each child.
At least 200 randomly selected cells were scored by fluorescence microscopy for each cell line under each condition.
DOI: http://dx.doi.org/10.7554/eLife.02565.004 'Baseline': Sputum smear microscopy for each diagnostic attempt, with liquid-media TB culture only to evaluate smear-positive cases with a history of previous TB treatment for drug resistance.
Each episode of symptomatic malaria was treated and the patient followed up at day 7. Parasitological cure was assessed by microscopy for each symptomatic malaria episode on day 7.
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To overcome these limitations we developed a protocol using triplicate slides from each specimen before and after bleach-sedimentation to characterise effects on smear-microscopy for each specimen.
We also present experimental results of high-resolution transmission electron microscopy (HRTEM) for each annealing step.
The extent of newly formed bone around the implant was measured from 200× fluorescence microscopy images for each type of implant.
Figure 4 shows laser scanning confocal microscopy images for each fluorescent dye and a corresponding brightfield image.
Dual-color fluorescent signals were visualized under fluorescence microscopy, and for each sample, at least 300 interphase nuclei were analyzed and scored by two independent investigators.
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