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[168] NaYF4 NPs modified with different compounds 94 550 nm 62.5 and 125 μg/ml; 24 h HaCaT Human skin fibroblasts MTT assay; confocal microscopy; fluorescent microscopy The NPs coated with polyethyleneimine (PEI), poly(lactide-co glycolide) (PLG), and PLG + dextran sulfate are the most toxic (52, 61, and 72% viable cells, respectively).
This conclusion was derived from in vivo two-photon laser scanning microscopy, fluorescent microscopy and measurements of the ISF volume comparing awake, asleep and anaesthesized animals.
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The fluorescence intensity was measured using fluorescent microscopy.
Fluorescence signals were analyzed by fluorescent microscopy (Nikon, Tokyo, Japan).
Autophagy was quantified by quantification of EGFP-LC3B puncta per cell using fluorescence microscopy, using an Olympus IX51 fluorescent microscopy system at 60× magnification.
The results from scanning electron microscopy and fluorescent microscopy showed a notable cellular growth and favorable morphological features.
Proliferation of different cell types like fibroblast and cardiac cells was analysed by scanning electron microscopy (SEM) and fluorescent microscopy.
X-ray diffraction (XRD), scanning electron microscopy (SEM) and fluorescent microscopy analysis confirmed biomimetic mineralization and SF coating on microspheres.
The CNPs were characterized by transmission electron microscopy, optical fluorescent microscopy, fluorescent spectrophotometry, fourier transform infrared spectrophotometry and ultraviolet-visible spectrophotometry.
The as-prepared nanoprobes and pure FMNPs were characterized by transmission electron microscopy, photoluminescence (PL) spectrometry, and fluorescent microscopy.
This unique self-fluorescent unimolecular micelle exhibited excellent photostability and low cytotoxicity, making it an attractive bioimaging probe for NP tracking for a variety of microscopy techniques including fluorescent microscopy, confocal laser scanning microscopy (CLSM), and two-photon microscopy.
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