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Cryo-transmission electron microscopy experiments detected F127 micelles, both embedded within PEGylated fibrinogen hydrogels and in solution.
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In inhibition experiments detected by confocal microscopy, the cells were preincubated with filipin (1 µg/ml) for 30 60 min, followed by baculovirus binding on ice for 30 60 min and transduction with or without drugs for 6 24 h at 37°C.
Consistent with our microscopy experiments, PARA was detected in P. aeruginosa cultivated in the presence of B. thai, but not in monoculture or with B. thai ∆tssM-1.
Confocal laser scanning microscopy experiments showed that ARH could be used to detect Fe3+ in living cells.
Confocal laser scanning microscopy experiments showed that rhodamine B-2-aminobutenedioate can be applied to detect acidic pH variations in living cells with a turn-on signal.
Fluorescence microscopy experiments further demonstrate that RBDPA can be used as a fluorescent probe to detect Al3+ in living cells.
For fluorescence microscopy experiments, PE-Texas Red (Invitrogen, Carlsbad, CA) labeled anti-GXM IgG mAb 18B7 was used to detect C. neoformans capsule.
In electron microscopy experiments with immuno-labelling of Ure2 aggregates (data not shown), formation of pores within the membrane could not be detected, whereas this has been reported for Aβ, α-synuclein, IAPP, polyglutamine, and PrP [55] [61].
The microscopy experiments in Figure 8 and 9 were performed as above except that the secondary antibodies goat anti-rabbit AlexaFluor 555 and goat anti-mouse AlexaFluor 488 were used to detect USP7 and PML, respectively.
All microscopy experiments were performed at the Cross Cancer Institute Cell Imaging Facility.
All microinjection and microscopy experiments were done at room temperature.
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