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A detailed electron microscopy examination was performed to study the microstructure of the FGMs.
Additionally, scanning electron microscopy examination was employed to characterize the microstructure of the fractured surface.
The microscopy examination was performed using a confocal laser scanning microscope (Leica TCS-SP5, Leica Microsystems Inc., Buffalo Grove, ILL, USA).
Transmission electron microscopy examination was performed as described [52].
All smear microscopy examination was performed by the auramine-O-phenol fluorescence method.
Light microscopy examination was performed on samples stained by H & E, PAS, Jones and Masson's trichrome.
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The Brunauer Emmett Teller (BET) surface areas and pore size distributions of the thin films coated onto the covering slides for microscopy examination were obtained from their hexane adsorption/desorption isotherms.
Since detection of the parasite in Giemsa-stained blood smears is the technique that has been traditionally used for diagnosis of piroplasmosis, whenever possible RLB and microscopy examination were performed in parallel.
An MTT reduction assay and fluorescent microscopy examination were used to evaluate cell survival, whereas the type of cell death was determined using LDH release assay, key signature of necrosis, and the expression level of apoptotic protein Caspase-3 and the antiapoptotic gene Bcl-2.
For verification transmission electron microscopy examinations were performed to determine the structural quality.
X-ray powder diffraction, X-ray tomography and scanning electron microscopy examinations were applied to study the conversion process.
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