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In the present study, different staining and microscopical techniques, including fluorescence microscopy, epipolarization microscopy of immunogold-silver, and absorbance microdensitometry were compared concerning suitability for quantitative evaluation.
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Applicability of the different techniques was tested by anti-CD45 immunostaining of leukocytes within rat lung tissue detected by immunofluorescence, immunogold-silver epipolarization microscopy, as well as alkaline phosphatase-based Vector Red absorbance or fluorescence measurement.
Expansion Microscopy.
With these in hand, they developed single-molecule microscopy.
Among the techniques he uses is electron microscopy (EM).
One way of looking at the fine structure of synapses is electron microscopy.
"If you don't have that, you don't have your microscopy," Dr. Weiss said.
"Their groundbreaking work has brought optical microscopy into the nano-dimension," the Nobel jury said.
Modern technologies — advances in microscopy, histological staining, biopsies, X-rays, computed tomography (C.T).
Using high-resolution microscopy, Currie's team filmed HSCs as they formed inside zebrafish embryos.
Using advanced cryo-transmission electron microscopy, they said, they can see in thousand-fold greater detail.
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