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These methods include fluorescence and luminescence resonance energy transfer, single-molecule bleaching analysis, intensity measurements, colocalization microscopy, electron transfer, and bimolecular complementation analysis.
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As an alternative to caged fluorophores for super-resolution microscopy, on- and off-states have been engineered by controlling the photophysics of fluorophores by electron transfer reactions.
This electron transfer event completes the circuit.
Scanning electron microscopy, fourier transfer infrared spectroscopy, X-ray diffractometry diffraction and X-ray photoelectron spectroscopy indicated the blending of PVA and sericin promoted the formation of hydrogen bond network between sericin and PVA, thus enhanced the mechanical performance and the stability of sericin, as well as the hygroscopicity and water retention capacity.
The samples were then prepared for electron microscopy and transferred to a Zeiss Gemini 1530 FEG SEM (Gloor Instruments AG, Uster, Switzerland).
The absolute coordinates of structures identified and located by cryo-light microscopy are transferred to the electron microscope via a Matlab-based user interface.
transfer electron microscopy.
Electron microscopy showed no electron dense deposits or organized deposits.
This procedure takes into account the most relevant physical parameters of cryo-electron microscopy including both contrast transfer function and camera factors.
Electron microscopy revealed granular electron-dense deposits.
The morphological of nanocomposite was characterized by scanning electron microscopy (SEM), Fourier transfer infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA).
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