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It is shown that acoustic imaging with single spherical lens is better suited to study bond quality compared to thermal microscopy due to its better detection capabilities.
Structural analyses of the cytoskeleton are especially demanding, because cytoskeletal networks are unresolvable by light microscopy due to their density and intricacy, whereas their proper preservation is a challenge for electron microscopy.
It is difficult to obtain useful fluorescence information from coals and source rocks of high maturity by means of conventional fluorescence microscopy due to the low fluorescence intensity of the macerals.
Deconvolution algorithms are widely used in conventional fluorescence microscopy, but they remain difficult to apply to deep imaging systems such as confocal and two-photon microscopy, due to the practical difficulty of measuring the system's point spread function (PSF), especially in biological experiments.
It could be used to target mitochondria under two-photon laser confocal microscopy, due to the energy of laser irradiation gained from the absorbed photons to be dispersed as excess heat to the neighboring particles and thus to induce their fusion, compared with free complex.
The separated cells were monitored by fluorescence imaging microscopy, due to the strong luminescence of these nanocomposite particles [36].
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Stereological analysis made in electron microscopy confirmed these results, although the total number of fibers quantified was significantly higher compared to light microscopy analysis, due to the very small size of some fibers that can be detected only in electron microscopy.
Two reference standards were used to assess the performance of the RDTs: microscopy and, a combination of microscopy plus nested PCR for slides reported as negative, on the assumption that negative results by microscopy were due to sub-patent parasitaemia.
With nanoparticles and cultured cells, it is difficult to determine how much of the loss of nanoparticles observed by confocal microscopy is due to this natural dilution, as compared to other sources of loss.
However, the fraction of those nanoparticles in different aging states is difficult to be analyzed only by transmission electron microscopy (TEM) due to the limitation of two-dimensional observation.
Such projections were not detected by laser scanning confocal microscopy, likely due to the longer acquisition times necessary for each xy frame.
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