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This technique is useful in practice given that optical and atomic force microscopy do not provide unambiguous information about crystal orientation.

Previous studies using electron microscopy do not apply directly to normal amniotic membrane because the specimen preparation procedures involve fixation, dehydration and chemical staining.

Analysis by flow cytometry and confocal microscopy do not allow confirmation that the fluorescence within the cell is from intact CPMV particles.

In addition, some assays, such as microscopy, do not distinguish between species.

Standard fixation protocols for electron microscopy do not preserve the glycocalyx, but newer fixation techniques have allowed the demonstration of a glomerular endothelial glycocalyx of 200 400 nm in thickness [ 33, 34].

The ability to quickly assess and correct for cross-talk is of vital importance, because the most widely used commercial setups that combine FCS and confocal microscopy do not routinely offer fast alternating excitation schemes during FCS acquisition.

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A post-test material characterization by electron microscopy did not show any significant degradation in the tubular SOC cell microstructure during the high pressure operation at 8 bar.

Analysis of samples by scanning electron microscopy did not reveal any cracks in the deposited layer formed during a sample immersion in water.

However, because of their small size and their lack of distinctive morphological features, traditional microscopy does not allow the detection of fungal zoospores in the field.

The SYTO 9 stain used for confocal microscopy does not affect the spectral signatures collected using STXM as the concentration was quite low.

However, because of their small size and their lack of distinctive morphological features, traditional microscopy does not allow the detection of chytrids, particularly of zoospores which have probably been misidentified as phagotrophic flagellates in previous studies.

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