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These elevations of LC3B-II and BACE1 levels by immunoblot and immunofluorescence microscopy corroborate our electron microscopy data demonstrating the accumulation of autophagosomes and BACE1 in dystrophic neurites, and may reflect either impaired clearance of autophagosomes, increased induction of autophagy, or a combination of both in the 5XFAD brain.
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Preliminary microscopy data demonstrated the ability of these polymers to form non-spherical domains of the B and C blocks.
Amid HCN4-positive cardiomyocytes, fluorescence and electron microscopy data demonstrated a dense distribution of nerve fibres immunoreactive for ChAT and TH.
Confocal microscopy data demonstrated that cholesterol-siRNA spread deep in the tissue and was present in the cytoplasm of almost all the liver and tumor cells.
Both fluorescence microscopy and electron microscopy data demonstrate that presence of contacts between cells facilitates the formation of Cx43 positive double-membranes, i.e. gap junctions (Fig. 1).
The X-ray diffraction (XRD) and transmission electron microscopy (TEM) data demonstrate that the core of this catalyst is a Pt particle, and the shell or completely alloyed nanoparticles are composed of a PtPb alloy with an hcp structure.
The X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) data demonstrate that a thinner SEI with less inorganic degradation products can be formed on the surface of the LiMn2O4 cathode due to SDPN.
Scanning ion conductance (patch clamp) microscopy data have demonstrated KATP channels to be organized in small clusters and to be anchored in the Z-grooves (t-tubular openings) of the sarcolemma [ 24].
This distribution is in line with previous fluorescence and electron microscopy data, which demonstrate the polar localization of PilQ (Seitz and Blokesch, 2013) and pili (Salzer et al., 2014c).
Together, these data demonstrate that spectral imaging microscopy can directly detect PCNA ubiquitination by FRET between CFP-Ub and mRFP-PCNA at the level of whole cells.
These data demonstrate that the study of these multi-component membrane inserted complexes within their native lipid environment by electron microscopy can identify extra components and/or structures which are lost during purification.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com