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The microstructure of the SEI layer, characterized using high-resolution transmission electron microscopy, consisted of an amorphous structure incorporating crystalline domains of ∼5 20 nm in size.
At 1 h, 1 and 2 days after leaf spraying, the leaves were examined for the presence of fluorescent bacteria using CLSM equipped with an Ar Hg laser (Leica TCS SP2, Leica, Wetzlar, Germany); the filter sets for fluorescence microscopy consisted of a 458-nm band-pass excitation and a 520 560 nm emission.
The structure of the Ti Fe Co alloys (at Fe/Co ratio >1) studied by X-ray diffractometry and scanning electron microscopy consisted of an ordered Pm¯3m Ti(FeCo) compound and a disordered body-centered cubic Im¯3m β-Ti solid solution.
Controls for light microscopy consisted of omitting the primary antiserum and secondary antibody separately.
Culture yielded colonies from 87 of 110 samples (79.1%), which on dark-field microscopy, consisted of spirochetes (Supplemental Table 4).
A general method for confocal microscopy consisted of plating 30 000 cells, MCF7 or WM115 in glass bottom dish with 300 μL RPMI medium and α-MEM medium for MCF7 and WM115 cell lines, respectively.
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As shown in Fig. 1, the schematic of our CARS microscopy consists of a laser source and a beam-scanning microscope.
Images were visualised using Perkin Elmer Ultraview ERS Suite v 3.0.0 and confocal microscopy consisting of Zeiss Axiovert 200 inverted fluorescence microscope (x40 oil objective) equipped with 14 bit ECCD camera and argon and krypton gas excitation lasers at 488 nm and 568 nm.
Instead, they support a model in which the asymmetric ParA cloud observed by epifluorescence microscopy consists of sparse ParA-ATP dimers (or small oligomers) bound to the chromosome away from the ParB/ parS complex.
Sample preparation for transmission electron microscopy (TEM) consisted of placing a volume of 0.5 μL QDs on TEM copper grids (400 mesh) with a holey carbon film.
Light microscopy samples consisted of fragments fixed immediately in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2, 3 h, 4°C), washed in 0.1 M cacodylate buffer, then postfixed in OsO4 and embedded in Epon.
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