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Here we report a microscopy configuration with an additional high spatial resolution fast camera (512 by 512 readout), which makes it possible to record both fast and slow events in small (close to diffraction limited) objects, such as varicosities in a neuronal network.
In this study we report the successful development of a microscopy configuration based on a widefield approach with a dual camera system that allows recording images both at fast (up to 2000 Hz) and standard (2-10 Hz) frame rates with sufficient spatial resolution.
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For optimization of the transmitter path for our system using the computational model, the optimum value for fill-factor, h, of the four confocal microscopy configurations: (1) full-pupil point-scanning, (2) full-pupil line-scanning, (3) divided-pupil point-scanning, and (4) divided-pupil line-scanning are 0.89, 1.02, 0.66, and 0.52 respectively.
TVD was calculated by adding the lengths of all detected capillaries calibrated to a pixel size of 2.8 μm given by the microscopy hardware configuration used, divided by the area of the field of view of 1.78 mm2.
A dual gold-copper microelectrode (diameter 25 μm) was fabricated to be employed in a scanning electrochemical microscopy (SECM) configuration, and was used in feedback mode to both modify (write) and analyze (read) a substrate.
DCEs are employed in a scanning electrochemical microscopy (SECM) configuration, and their use for both approach curves and imaging is considered.
The heterogeneous precipitation of the S (Al2CuMg) phase along dislocations is investigated using high-resolution transmission electron microscopy various configurations of the S precipitate group form according to the initial dislocation morphology and no more than two S variants coexist along a single dislocation line.
We report a Fourier-analysis-based computational model of confocal microscopy for six configurations.
In this paper, we report a Fourier-analysis-based computational model of confocal microscopy for six configurations.
In the proposed technique, however, the complex fields are provided by digital holographic microscopy (DHM) in transmission configuration [ 17] as shown in Fig. 2(a).
Using an original numerical simulator of ILIDS images, we propose and discuss the performances of different ILIDS configurations for microscopy, volumic 3D droplet characterization, and fringe frequency calibrations.
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