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Finally, samples were mounted for fluorescence microscopy by using ProLong antifade kit (Invitrogen).
Finally, cells were washed and mounted for flurescence microscopy by using ProLong antifade kit (Invitrogen).
Recently, activated Arf1 has also been detected in punctuate structures close to/at the plasma membrane by TIRF microscopy by using the Arf-binding domain (ABD) of ARHGAP10 fused to GFP as a specific sensor [10].
This analysis was performed using confocal images from coronal sections at similar rostro-caudal levels (from bregma −1.70 mm to −3.00 mm) obtained with a Leica TCS SP5 laser confocal microscopy by using the Leica Application Suite (Advanced Fluorescente Lite 1.8.1).
A subset of ticks was dissected, and midguts were screened for spirochetes by fluorescent microscopy by using a polyclonal antiborrelial antibody (18 ).
Assessments of serum IgG anti-double-stranded DNA (anti-dsDNA) antibodies were carried out by immunofluorescence microscopy by using Crithidiae luciliae as a source of antigen.
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Changes in the structure of actin microfilaments were evaluated using the method of fluorescent microscopy and by using an LSM 780 (Carl Zeiss, Oberkochen, Germany) confocal microscope.
The urine analysis was carried out by microscopy and by using UroColor™10 Dip Sticks (Standard Diagnostics, Kyonggi-do, Korea).
Free-swimming larvae were processed for transmission electron microscopy (TEM) by using the high-pressure freezing (HPF) technique.
The nanoparticles were imaged by transmission electron microscopy (TEM) by using a Tecnai G2 F30 transmission electron microscope at 300 kV (FEI Corporation, Hillsboro, OR, USA).
Our results, through immunoflourescent confocal microscopy experiments by using anti-V5 antibodies under permeabilised conditions, showed mainly cytoplasmic cellular CSNK2B protein distribution, in agreement with previous data [ 21].
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