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To reconcile this with our current observation of nanoglobule-based fibrillation, we performed sub-micron resolution compositional investigation using stimulated emission depletion (STED) microscopy by staining crude slime with a lipophilic probe (m-Cling)21 and an isothiocyanate protein stain (Rhodamine b).
The viability of B. subtilis was assessed by using fluorescent microscopy by staining cells with Propidium iodide (PI) at 20× magnifications.
This observation was further confirmed by confocal microscopy by staining DC with anti-CD28 antibody.
Vacuoles were visualised under fluorescence microscopy by staining with monodansylcadaverine (MDC) as distinct dot-like structures.
The success of these treatments were monitored using fluorescence microscopy by staining the cells with 4′,6-diamidino-2-phenylindole.
The presence of the biofilm was confirmed by confocal laser scanning microscopy by staining the biofilm matrix with Wheat Germ Agglutinin (WGA -Oregon green 488.
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Cell transformation was assessed either by phase‐contrast microscopy or by staining with the Giemsa solution.
Trunk and tail morphology, and the shape of muscle segments, were scored by bright-field microscopy or by staining actin filaments with phalloidin (described below).
Further ultrastructural evidence of mast cells in tissues from failed joint interface membranes was shown by transmission electron microscopy, and detection by staining after magnetic activated cell sorting.
During cell culture, in situ imaging and morphological characterisation of cells was assessed using brightfield light and/or fluorescence microscopy, and later confirmed by staining of fixed cells using immunofluorescence microscopy.
Next, to investigate the photothermal effect in vitro, the fluorescence microscopy images were obtained by staining the live cells with calcein AM, which can emit strong green fluorescence in live cells.
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