By removing out-of-plane probes using HiLo microscopy with PTM, we were able to more accurately measure the mechanical properties of individual cell layers.
We examined the reversibility of Plk1 inhibition by removing the drug and following cytokinesis and anaphase B recovery by timelapse microscopy.
This was done by removing the tumors and visualizing the autophagy response within the tumor cells by electron microscopy (Fig. 9 ).
Actin filament motion was observed by epifluorescence microscopy after removing the donor.
The membrane containing invading cells was fixed using methanol, stained with Wright-Giemsa, and invasion was quantified by light microscopy after removing the non-invading cells on the upper side of the membrane with cotton swabs.
The intact chloroplast layer, identified by microscopy, was removed, transferred to SDS-PAGE cocktail, boiled for 10 min and stored at -20°C.
Owing to higher resolution, electron microscopy largely removes such ambiguity.
Samples for cell counts (1 mL for HC treatments, 10 mL for LC treatments) by fluorescent microscopy were also removed at the beginning and end of the experiment, and immediately preserved with 2% formaldehyde and refrigerated until processing.
After squashing, the slides were then scanned for good chromosome spreads at prophase or metaphase stages with phase contrast microscopy, then slips were removed by immersing slides into liquid nitrogen for 10 15 min, and the air-dried slides were stored at room temperature until use.
After 2 weeks, horizontally cut sample biopsies were removed, and the numbers of hair follicles were counted under high field microscopy by a specialist blinded to the treatments.
