Sentence examples for microscopy by postfixing from inspiring English sources

Samples were treated for electron microscopy by postfixing the cells for 1 h in 1% osmium tetroxide in phosphate buffer pH 7.4 containing 1% CaCl2.

For ultrastructural observation, cell monolayers were fixed in 2.5% glutaraldehyde (Electron Microscopy Science, Pennsylvania, USA) and in 0.1% cacodylate buffer (Electron Microscopy Science), postfixed in 1% osmium tetroxide (Electron Microscopy Science) and then treated with 1% tannic acid (Acros Organics, New Jersey, NJ, USA).

For electron microscopy, the specimens were postfixed with 1% OsO4/water for 2 h on ice, washed with PBS and water, and en bloc contrasted with 1% uranyl acetate in water.

For light microscopy (LM) 7 μm sections were mounted in aquamount; for electron microscopy 60 μm sections were postfixed in 1% OsO4.

For electron microscopy, glutaraldehyde-fixed specimens were postfixed with 1% osmium tetroxide (Serva, Heidelberg, Germany) and embedded in araldite (Serva).

Animals were perfused transcardially under anesthesia with 0.9% normal saline followed by 4% w/v paraformaldehyde in 0.1 M phosphate buffer, and postfixed by immersion at 4 °C overnight.

Next, they were washed thrice in 0.2M cacodylate buffer (pH 7.2 7.4) and postfixed by 0.1% OsO4 in the same buffer for 1 h, at room temperature.

The brains were removed from the skull and postfixed by immersion in the same fixative overnight at 4°C.

The brain was removed, cut along the midline and postfixed by incubation overnight in 4% paraformaldehyde at 4°C.

Mice were perfused with 3% paraformaldehyde at 3 days, 2 weeks, or 6 weeks after surgery, and brains were postfixed by immersion overnight.

The presence of PrPTSE was detected by Western- and postfixed frozen tissue blotting, while the seeding activity of PrPTSE was revealed by protein misfolding cyclic amplification (PMCA).