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Microscopy at various length scales (transmission confocal laser scanning and cryo-scanning electron microscopy) and static light scattering measurements revealed emulsion microgel particles of 5 50 μm diameter.
Donor tissue was labelled with the non-diffusing fluorescein derivative carboxyfluorescein diacetate succinimidyl ester, later visualized by fluorescence microscopy at various timepoints during survival and by a sensitive whole-mount immunocytochemical protocol after fixation.
Droplet size, microscopy at various length scales (confocal, electron microscopy), ζ-potential and kinetics of fatty acid release were used to assess how the presence of CNCs impacted the microstructural stability of the emulsions in in vitro duodenal conditions (pH 6.8, 37 ○C).
Microscopy at various length scales (confocal laser scanning microscopy, scanning electron microscopy) and static light scattering measurements revealed a decrease in particle size (100 10 μm) with lower turbulent mixing time (ca. 4 × 10−4 s) and lower concentrations of calcium ions (0.1 0.02 M).
The "wounded" areas were photographed by phase contrast microscopy at various time points (0, 3, 6, 8, 9, 10, 12, 21, 22, 23 and 24 h after scraping) depending on the cell line.
For semi-thin sections, the embedded samples were sectioned, transferred to a microscope slide, stained with new fuchsin and crystal violet, mounted in Entellan (Merck, Germany) under a cover slide, and studied by light microscopy at various magnifications.
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The surface micro-topography of corrosion scale was observed by scanning electron microscopy (SEM) at various alternating injection frequencies (F = 0, 1, 2, 4 and 6).
The internalization of GF into KB cells was also confirmed by confocal microscopy performed at various depths (Figure 9), showing a weak signal at the top (Figure 9a) and bottom (Figure 9c) of the cells but intense signals in the middle (Figure 9b).
After the membranes had dried, they were then mounted onto microscope slides ready for optical microscopy investigation at various magnifications (4x, 10x, 20x, and 40x).
Cy-3 fluorescence signals were observed under the fluorescence microscopy (Olympus) at various time points (1, 2, 4 and 6 h post transfection) after removal of the old medium, PBS wash and addition of fresh medium.
Conventional and high-resolution electron microscopy was performed at various depths of the deformed layer.
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