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Chin R. and Sinclair R. Developing remote microscopy at a university user facility.
CFZ's macrophage-targeting chemical and structural forms were detected with PA microscopy at a high contrast-to-noise ratio (CNR > 22 dB) as well as with macroscopic imaging using synthetic gelatin phantoms.
There is large variation between studies on the probability of detection by microscopy; at a qPCR-detected parasite density of 100 parasites per µl, the median probability of being detected by microscopy is 29.7%, with a range of 3.8−69.7%.
W. Chao, B. D. Harteneck, J. A. Liddle, E. H. Anderson, and D. T. Attwood, "Soft X-ray microscopy at a spatial resolution better than 15 nm," Nature, vol. 435, pp. 1210-1213, June 2005.
The focused spot size of microlenses after thermal reflow was measured by optical microscopy at a fixed focal distance and the geometry of microlenses was evaluated by atomic force microscope.
The sample structure was examined by means of transmission electron microscopy at a voltage of 100 kV.
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Spinning disc confocal microscopy at 40x (A-D ) and TIRF microscopy at 100x (E H ) of ATDC5 cells expressing TβRII-mEmerald and integrin α2-mCherry demonstrate a loss of TβRII depleted regions (A,B ), TβRII peripheral ring formations (E,F ), and integrin α2 developed adhesions (C,D,G, H) on poly-l-lysine relative to collagen II-coated substrates.
The real-time imaging for caspase activation and Mito-DsRed fluorescence was carried out using confocal microscopy at an interval of 15 min as described in 'Materials and Methods'.
At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore.
The sample structure was examined by means of transmission electron microscopy at an accelerating voltage of 100 kV.
All of the TEM images were recorded using a JEOL JEM-2100 electron microscopy at an accelerating bias voltage of 200 kV.
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