Confocal microscopy assays, using NMDA, AMPA, and DHPG agonists in isolated astrocytes and pinealocytes, revealed increased intracellular calcium content in both cell types for all the agonists used.
To study the contact inhibition of the cell lines expressing different levels of Arl2 we have evaluated their respective proliferation rates at confluence using phase contrast microscopy, MTT assays and flow cytometry analyses.
At 3 4, 15 and 26 weeks of age, oral glucose tolerance tests were performed, and pancreas tissue was collected for electron microscopy, enzyme activity assays and islet isolation.
Intestinal parasites were diagnosed by microscopy, stool antigen assays and PCR, and bacteria by culturing methods.
Cellular compatibility was determined using cell viability assays and microscopy imaging, with the results showing that the nanofibrous membranes support 3T3 cell viability and attachments.
DA planned the atomic force microscopy and cell toxicity assays and participated in preparing the manuscript.
To address this issue, we performed biochemical pull-down, in situ immunofluorescence and in vivo microscopy assays (Figs. 3 and 4).
Correlating the results obtained via quantitative and microscopy assays, we can generally conclude that the ratio between live and dead cells is strongly positive for all the studied bioconstructs, reflecting good biocompatibility of these materials.
Ethanol-peracetic acid– and supercritical carbon dioxide treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays.
As expected, the iRGD motif mediated specific targeting to tumor and endothelial cells and enhanced cellular uptake of the NGs, as demonstrated by flow cytometry and confocal microscopy assays.
