Sentence examples for microscopy assay was from inspiring English sources

To efficiently and accurately quantify the interactions of bacteria with mammalian cells, a reliable fluorescence microscopy assay was developed.

The microscopy assay was automated using a high-content screening instrument programmed to detect and quantitate punctate EGFP-LC3 fluorescence.

In this study, immunofluorescent microscopy assay was applied to investigate the cellular distribution pattern of IFT25 together with complex B subunits IFT46 and IFT27 as controls.

To consolidate that PTEN is an intramitochondrial protein, immunoelectron microscopy assay was performed with an antibody specifically against PTEN with non-related IgG as a negative control.

Live/dead fluorescence microscopy assay was performed to evaluate ASCs viability and proliferation within the 3D culture systems, using Live/Dead kit (Invitrogen, Life Technologies, Foster City, CA, USA).

The concentrations of Ni nanoparticles cocultured with cells for optical microscopy assay were 3.12, 12.5 and 50 μg/mL, respectively, and cultured in the incubator for 72 h at 37 °C with 5% CO2; while the concentration of Ni nanoparticles cocultured with cells for AFM assay was 12.5 μg/mL and incubated for 6 h at 37 °C with 5% CO2.

Transfection and fluorescence microscopy assays were fulfilled as shown in our previous studies [ 23, 53].

Consequently, patients with EVD returning from areas endemic for malaria should receive empirical malaria treatment if neither molecular or microscopy assays are available or if concerns on malaria are raised.

For fluorescence microscopy, the identical assay was performed with the exceptions that Alexa Fluor® 555-labelled transferrin (Invitrogen; 20 μg/ml in serum-free Dulbecco's modified Eagle's medium) was used and cells were not detached before fixation.

After 24 h in the presence or absence of either purified p61-Sema3E or Uncl-Sema3E (2 µg/ml each), the wells were photographed using phase-contrast microscopy, and the assay was quantified by counting tube bifurcations in seven independent fields per each experimental condition.

In order to further investigate the disulfiram effects seen by microscopy, a flow cytometry assay was developed to enable quantitative comparison of intracellular zinc levels in disulfiram and sodium pyrithione treated cells.