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BrUTP incorporation and microscopy are detailed in the Supplementary data.
Standard methods used for strain construction, western blotting, and microscopy are detailed in the Supplemental Experimental Procedures.
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Customizable scalable methods, big data workflows, and visualization for scanning and electron microscopy data are detailed in the following sections.
The overall sensitivities, specificities, NPV and PPV for the three RDTs, evaluated against microscopy and microscopy and PCR are detailed in Table 2.
Protocols for immunofluorescent staining, confocal microscopy, image acquisition and analysis are detailed in [9], [21], [28].
Here, single-molecule imaging, smart fluorescent probes, two-photon microscopy, Förster resonance energy transfer (FRET) and super-resolution microscopy are discussed in detail.
The system lay-out and properties (for reflectance microscopy) are described in detail elsewhere [ 32].
LC3B and ATG12 immunofluorescence staining and quantifications as well as time-lapse microscopy are described in detail in Supplemental Experimental Procedures.
Fluorescence microscopy was performed as detailed by Alayari et al. [9].
The images from electron microscopy were not sufficiently detailed to reveal the organization of the proteins within these modules.
Raman spectra and transmission electron microscopy are used to investigate details of the modified layer.
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