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Microscopy approaches have also led to conflicting conclusions, with some studies supporting cytoplasmic uncoating, whilst others have suggested later, NPC associated, uncoating [ 5,6 8].
Over the last decade, several so-called super-resolution microscopy approaches have been developed; in this paper we explore the potential of such approaches for the direct visualization of cellulose.
While several cost-effective microscopy approaches have been recently developed that are capable of making microscale measurements, including, e.g., imagers that are integrated with mobile phones or are folded out of paper, in this manuscript, we will focus on the optical designs that enable nanoscale measurements and sensing.
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The video microscopy approach had a technical advantage of enabling a complete experiment to be run without physical disturbance (as incubator conditions were present during recording).
Studies using immunofluorescence microscopy and pharmacological approaches have shown that microtubules and/or actin filaments are involved in many of these events through the generation of intracellular movement of cell components or entire protoplasmic contents and the spatial control of cell activities in specific areas of the giant cells.
The mechanisms behind the long-range yet cis-specific localization and silencing activities of Xist have long been enigmatic, but genomics, proteomics, super-resolution microscopy, and innovative genetic approaches have produced significant new insights in recent years.
Recent studies performed using 3C (chromosome conformation capture -based methods, FISH (fluorescapture -basedhybridization) coupled with confocal methodsopy, and other experimental approaches have revealed that the spatial interactions oFISHstant genomic elements within a fluorescenceosome are specinic and functionally relevant.
Electron microscopy and cell biological approaches have shown that the GF-PSI synapse is also a mixed electro-chemical synapse.
Although histology has traditionally been taught as a lecture- and microscopy-based course, new approaches have recently been used to emphasize self-learning processes such as virtual microscopy, team-based peer teaching and learning, and clinical-histologic conferences [ 36- 38].
Single-molecule methods, such as atomic force microscopy (AFM) and fluorescence-based approaches, have provided insights into how RNAP locates its promoter and unwinds the DNA duplex.
Nevertheless, except for transmission electron microscopy (TEM) observations [15], few experimental approaches have been performed to examine this phenomenon in detail.
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