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Optimized smear microscopy approaches for children should be explored further.
Other species-specific differences in parasite pathology, such as differences in replication rate and damage done to midgut tissue, in addition to molecular evidence (which remains to be verified via microscopy approaches) for differences in host tissue distribution, may also contribute to N. ceranae's alleged greater virulence [ 8, 14, 15, 20, 21].
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Herein, we employed a comprehensive confocal microscopy approach for FRET studies in cells co-expressing the APPL1 and APPL2 minimal BAR domains as CFP and YFP fusions, which allowed us to determine FRET values in a single cell using three FRET methods.
Sprung and Weckhuysen refined the confocal fluorescence microscopy approach for single FCC catalyst particles, containing ZSM-5, and determined the size, distribution, orientation and amount of zeolite ZSM-5 aggregates within binder material.
Despite these limitations, the findings reported here suggest that smear microscopy approaches could be optimized for children and these are urgently needed.
To quantitate NK4 repression, we used the same microscopy approach as for assaying HGF induction.
Most 3D localization microscopy approaches use high NA oil-immersion objectives for surface confined total internal reflection fluorescence (TIRF) irradiation.
Markers (e.g., cluster of cells and corner of the silicon nitride window) were employed to identify the target single cells for imaging with multiple microscopy approaches.
Over the last decade, several so-called super-resolution microscopy approaches have been developed; in this paper we explore the potential of such approaches for the direct visualization of cellulose.
Such procedures are not only essential for the iLEM, but also of great value to other correlative microscopy approaches.
Subcellular fractionation assays and microscopy approaches were used.
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