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Immunoblotting, immunofluorescence analyses and microscopy approaches are described in Supplementary Materials and Methods.
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Subcellular fractionation assays and microscopy approaches were used.
Confocal and electron microscopy approaches were used to define the localization of CB1 in the alBNST.
Confocal and electron microscopy approaches were used to identify the localization of proteins known to participate to eCB-mediated retrograde signaling in the striatum and the hippocampus [1], [25], [26], [27], [28], [29].
To gain insight into the precise location of free zinc within intracellular M. tuberculosis or the ctpC null mutant, electron microscopy approaches were used.
An original transmission electron microscopy approach was conducted, based on the use of a pseudo-environmental TEM holder that provides direct observations, on given zones of the samples, of the formation (in the case of zeolite) or not (in the case of alumina) of reduced silver particles upon dehydration of the material in air.
This 'systems microscopy' approach is well suited for parallel screening of cellular responses to numerous experimental perturbations [ 47].
To counter this problem, a confocal microscopy approach was used to measure the distribution of BODIPY-taxol in TS.
Those data showed a similar pattern of changes in FDB fibers using both approaches and for convenience the fluorescence microscopy approach was used in the current studies [17].
Another promising feature of a two-photon microscopy approach is the possibility to investigate sub-glomerular structures down to single neurons [ 7].
In the present study, a novel dual-modality iodine-labeled fluorescent probe (FADDI-096) was designed and synthesized, and a correlative microscopy approach was employed for quantitatively mapping the intracellular accumulation of polymyxin in single kidney tubular cells.
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