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Transfection was confirmed with fluorescence microscopy and western blot (CYP1A1).
Figure 4: Detectionof Z-ring formation using confocal microscopy and western blot analysis of E. coliJW0093.
TLR9 inhibitory oligonucleotides prevented expression of essential immediate early herpes gene products as determined by immunofluorescence microscopy and Western blotting.
Notably, all isolations contained 40 100 nm vesicles, and were positive for exosome markers (Alix, TSG101, HSP70) based on electron microscopy and Western blotting.
Supporting this hypothesis, immunofluorescence microscopy and western blot analysis revealed increased membrane localization and total expression of N-cadherin and vimentin in cells undergoing mesenchymal-like transition.
Here, we found that the addition of Kyn to B16 TRCs resulted in the translocation of AhR to the nucleus, demonstrated by fluorescent microscopy and western blot (Fig. 4a,b).
hCAREx8 expression was evaluated by immunocytochemistry (red fluorescence) using fluorescence microscopy and Western blot.
Fluorescent microscopy and Western blotting confirmed expression of P protein fused with GFP.
Their purity of exosomal fractions was assessed by analyses of transmission electron microscopy and western blotting.
Expression was confirmed with fluorescent microscopy, and Western blot analyses of lysates from transfected cells as previously described [27].
Individual clones were checked for expression of the NhttEGFP transgene by microscopy and Western blot analysis following induction with CuSO4.
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