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Some ears were processed for transmission electron microscopy and viewed in a Hitachi TEM as previously described [39].
Areas with suitable staining were prepared for electron microscopy and viewed on a Phillips CM10 transmission electron microscope.
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The slides were mounted and viewed by fluorescent microscopy and brightfield DIC microscopy; images were digitally recorded (Zeiss AxioCam) and further processed by 2D deconvolution software (Zeiss Axiovision).
Proliferation was first measured by counting mitotic bodies on paraffin-embedded tumour specimens stained using haematoxylin-eosin and viewed by microscopy.
The sections (thickness 500 nm) were cut (Leica EM UC6 microtome, Leica Microsystems, Wetzlar, Germany) and viewed under light microscopy.
Slides were washed three times in PBS and mounted and viewed using confocal microscopy.
Coverslips were then washed and mounted, together with a quenching agent (Citifluor), and viewed by fluorescence microscopy.
To visualize the tissue localization of 5-BP labeled proteins, OCT tissue sections were stained with fluorescently labeled streptavidin and viewed using fluorescence microscopy.
Stained cells were mounted in Prolong Antifade medium (Molecular Probes, Invitrogen) and viewed by epifluorescence microscopy (Axioplan with objective EC Plan Neofluar 40×/1.3 oil ph 3, Carl Zeiss AG, Zürich).
Slides were coverslipped and viewed with light microscopy.
Five-micrometer-thick sections were stained with hematoxylin and eosin and viewed by light microscopy.
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