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Two different peptides, YTA2 and YTA4, were designed and their intracellular delivery efficiency was characterized by fluorescence microscopy and quantified by fluorometry.
The preferential uptake of fluorescein isothiocyanate-labeled linear-dendritic nanoparticles into human MDA-MB-231 breast adenocarcinoma cells at temperature above the LCST (37 °C) was confirmed by confocal microscopy and quantified by flow cytometry.
The delivery of both a fluorescence marker (calcein) and antiviral drugs into cells has been achieved owing to a large extent of internalization of cationic liposomes (labelled by Lyssamine-Rhodamine PE or fluorescein-PE) as demonstrated by fluorescent microscopy and quantified by flow cytometry.
Cross-sectional area data was collected via phase-contrast microscopy, and quantified using the ImageJ software package.
Similar to the TE26 reporter, Cre mediated recombination will allow for EYFP expression which can be visualized by fluorescence microscopy and quantified by flow cytometric analysis.
After 6 hours in culture, we visualized epidermal sheets by immunofluorescent microscopy and quantified the number of dendrites per cell to determine cellular rounding after stimulation.
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We demonstrate the presence of ferroelectric domains in CH3NH3PbI3 by piezoresponse force microscopy and quantify the coercive field to the switching of the polarization of ferroelectric CH3NH3PbI3.
The distribution of nanoparticles was visualized by confocal laser scanning microscopy (CLSM) and quantified by high performance liquid chromatography (HPLC).
Uptake of SWCNHs by exposed crops and tobacco cells was confirmed by transmission electron microscopy (TEM) and quantified by microwave induced heating (MIH) technique.
Neutrophils were purified from heparinized whole blood on a Polymorphprep™ density gradient (AXIS-SHIELD, PoC AS) and surface expression of CXCR1 was detected by confocal microscopy Bio-Rad Laboratoriess) and quantified by flow cytometry (Coulter, 3000 cells/sample) as previously described [5].
Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay.
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