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This includes high throughput gene-to-structure determination, structure-based drug design, crystallography, NMR spectroscopy, cryo-electron microscopy and protein production.
The 3D static cultures (constructs) were examined qualitatively and semiquantitatively by light microscopy, immunofluorescence microscopy and protein immunoblotting.
We discuss the complementary approaches of high-resolution electron microscopy and protein crystallography to the structure determination of subunits of multimeric complexes.
Analysis of bacteriophage T5 by cryo-electron microscopy and protein sequence analysis reveals analogies with HK97 and T4 that suggest a mosaic of such connections.
The transcribing Pol IIp-CE complex catalyzes the first two steps of capping, and its analysis by mass spectrometry, cryo-electron microscopy, and protein crosslinking revealed the molecular basis for transcription-coupled pre-mRNA capping.
Polymers were complexed with plasmid DNA at pH 7.4 and the ability of the resulting particles to transfect cells was assessed via confocal microscopy and protein expression studies in tissue culture.
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Using 3D homology modeling, genetics, fluorescence microscopy and protein-protein interaction experiments, we provide data supporting the idea that Pmp23 is a bacterial lysozyme involved in the stability of the division machinery, revealing a new connection between cell wall metabolism and cell division.
They analyzed a variety of markers of kidney injury, including serum creatinine levels, kidney cells on microscopy, and proteins in urine.
Pathological destruction of the blood brain barrier (BBB) ultrastructure was assessed by transmission electron microscopy, and proteins involved in the MEK1/2/ERK1/2/p90RSK pathway were detected by immunofluorescence and Western blotting.
Studies on the mechanism of action (MOA) of these fluorotaxoids were performed by tubulin polymerization assay, morphology analysis by electron microscopy (EM) and protein binding assays.
Surprisingly, this massive phosphorylation of Rap1 is dispensable for telomere bouquet dissociation, as indicated by our live microscopy analysis and protein interaction studies of the phospho-mutants.
More suggestions(15)
microscopy and gene
ultrastructural and protein
microscopy and profilometry
microscopy and microscope
microscopy and laser
microscopy and energy
microscopy and X-ray
microscopy and tissue
microscopy and embryo
microscopy and cell
microscopy and imaging
microscopy and image
microscopy and sensing
microscopy and immunofluorescence
microscopy and deconvolution
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