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Confocal microscopy and mitochondrial fractionation were used to determine the importance of mitochondria for caspase-8 activation.
Using a combination of flow cytometry, confocal microscopy and mitochondrial fractionation, we demonstrate that NeuroD6 stimulates maximal mitochondrial mass at the lamellipodia stage, thus preceding axonal growth.
Immunogold electron microscopy and mitochondrial fractionation demonstrated that following IR, mitochondrial PKCε is localized within the mitochondria, on the inner mitochondrial membrane.
Using a combination of flow cytometry, confocal fluorescence microscopy and mitochondrial fractionation, we found that NeuroD6 sustains mitochondrial mass, intracellular ATP levels and expression of specific subunits of respiratory complexes upon oxidative stress triggered by withdrawal of trophic factors.
Using a combination of flow cytometry, confocal microscopy and mitochondrial fractionation, we found that NeuroD6 induced maximal increase of mitochondrial mass at the very first stage of neuronal differentiation, the lamellipodia stage.
Electron microscopy and mitochondrial subfractionation analyses confirmed that intra-mitochondrial PKCε levels are increased by IR in an HSP90-dependent manner and demonstrated that mitochondrial PKCε is localized at the matrix side of the IMM.
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To test this hypothesis, we used three distinct but complementary experimental approaches: flow cytometry, confocal fluorescence microscopy and mitochondrial-enriched fractionation.
These Tfam levels are in keeping with the maintained mitochondrial mass in serum-deprived PC12-ND6 cells assessed by flow cytometry, confocal microscopy and mitochondrial-enriched fractionation.
Analysis of larval Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described.
Mitochondrial PKCε location was examined by immunogold electron microscopy (EM) and mitochondrial subfractionation.
Mitochondrial ultrastructure in RGCs was examined by electron microscopy (EM), and mitochondrial functions were also tested.
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