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Suitable nanowire junctions with electrodes at the ends were located using optical microscopy and later probed with an Agilent 4155C Semiconductor Analyzer as the current forcing device.
During cell culture, in situ imaging and morphological characterisation of cells was assessed using brightfield light and/or fluorescence microscopy, and later confirmed by staining of fixed cells using immunofluorescence microscopy.
Each month a blood sample for microscopy and later genotyping was systematically collected.
During surveys, each child was examined by a physician, and a blood sample for hematology, the detection of malaria infection (microscopy), and later, genotyping was collected.
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To explore the response of cells' EGFP fluorescence intensity to the treatment of 5-AZA-CdR gradients in the TDQ experiment, the spontaneous fluorescence intensity of cells with increasing concentrations of 5-AZA-CdR (Table 1) was evaluated directly via microscopy imaging, and later by using flow cytometry measurement to find the percentage of positive fluorescent cells.
Tissues were dehydrated for transmission electron microscopy, infiltrated overnight and later embedded in epon resin [ 102].
Moribund larvae were collected from the rearing tanks and transported to the Fish Disease Research Laboratory at the University of Bergen in May 2013, where fish from three different tanks were processed for histology and histopathology (n = 14), and later transmission electron microscopy (n = 1).
The samples are solidified under the contactless ultrasonic treatment and later analyzed by electron microscopy and energy-dispersive X-ray spectroscopy (EDX).
EmGFP fluorescence was confirmed 24 h later by microscopy, and whole-cell protein extract was obtained by lysis in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS) with protease inhibitor cocktail (Complete, Mini, EDTA-free, Roche Molecular Biochemicals, Indianapolis, IN).
LSPCs containing 4 nmol of siRNA and liposomes and 40 nmol of peptide were injected into the tail veins of FVB mice and LSPC delivery assayed 24 hours later by fluorescent microscopy and FACS.
The phenotype of embryos among litters from Axd/+ intercrosses was examined by scanning electron microscopy (70 embryos) and light microscopy (>300 embryos), at developmental stages during and after the stage of spinal neural tube closure (E9.5 10.5 and later).
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