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Immunoprecipitation, electron microscopy and immunogold labelling were performed according to [49] and [50], respectively.
Transmission electron microscopy and immunogold labeling also revealed that the insulin receptor and caveolin-1 were recovered in the same vesicles in the endosome fraction (Fig. 4).
Third, electron microscopy and immunogold labeling confirmed that ARCβ and ARCγ associate with the cortical granule lumen, but not with underlying yolk platelets, cytosol, or ER.
Moreover, electron microscopy and immunogold labeling studies show the first physical evidence of mitochondria forming continuous structures with LC3-labeled autophagosomes.
Immunofluorescence microscopy and immunogold electron microscopy in tibialis anterior samples of the control and the overexpressing muscle confirmed specific localisation of PLIN5 to mitochondria and lipid droplets.
Again, the most significant finding is the evidence of nestin in the cell nucleus, which we detected using transmission electron microscopy and immunogold labelling.
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The resulting exosome pellet was resuspended in 10 ml sterile PBS and centrifuged again at 100,000×g for 70 min. The pellet was resuspended in 50 µl 2% paraformaldehyde and processed for transmission electron microscopy (TEM) and immunogold labeling for αB crystallin or processed for Western blot analysis.
Therefore, electron microscopy (EM) and immunogold labeling were performed on leaf tissue expressing ER-targeted GFP-ELP to further investigate this question.
We have studied the intracellular distribution of the proto-oncogene product pp60c-src in human platelets by immunofluorescence microscopy and by immunogold electron microscopy on frozen thin sections.
The hepatocellular carcinoma cell line Huh7 was treated with benzyl butyl phthalate (BBP), and then analyzed by total internal reflection fluorescence microscopy, confocal microscopy and double immunogold transmission electron microscopy.
These structural similarities, along with the polarized labelling seen by immunofluorescence microscopy (Fig. 1A) and immunogold EM (Fig. S3), indicate the complexes are chlamydial T3SSs.
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