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Bud scar counting: The number of bud scars was counted using microscopy and evaluated from flow cytometry data.
Each immunostained section was examined under light microscopy and evaluated by a senior pathologist (TY) according to the modified scoring method of Hugh et al (1999).
The sections were incubated with indicated primary and secondary antibodies and observed under an E2000U confocal microscope or fluorescent microscopy and evaluated using Image-Pro Plusoftwarere.
To analyze cellular damage to electronic microscopy and evaluated its possible relation with serum cardiac markers (troponin, MB-creatin phosphate kinase), and homodynamic data.
Tissues were processed for light and transmission electron microscopy and evaluated based on criteria described by Markwald (1968) and Veeramachaneni et al. (1986).
All samples were investigated by an experienced pathologist (blinded to the protocols) using light and electron microscopy and evaluated by a semiquantitative method.
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The morphology of molten blend samples collected at different locations was observed by scanning electron microscopy (SEM) and evaluated both qualitatively and quantitatively.
To demonstrate the biomechanical characteristics of our periosteal sheets, we have determined their stiffnesses with atomic force microscopy (AFM) and evaluated the expression of extracellular matrix (ECM) components specifically by both immunocytochemistry and a complementary DNA microarray technology.
We then measured the electronic states of the constituent elements on the irradiated surface by X-ray photoelectron microscopy (XPS) and evaluated the hydrolytic degradation properties (weight loss, media pH, and tensile strength) of the mesh in phosphate buffer solution.
The solids were characterized through X-ray diffraction (XRD), Raman and Mössbauer spectroscopy, nitrogen adsorption desorption isotherms, thermoprogrammed reduction (TPR) and thermoprogrammed desorption of CO2 (TPD-CO2), scanning electron microscopy (SEM) and evaluated in dry reforming of methane.
The assembled scaffolds were analyzed by scanning electron microscopy (SEM) and evaluated in terms of ceramic-polymer fitting, DBM infusion, and overall construct integrity (figure 2).
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