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Starch modification was analysed at different structural levels by differential scanning calorimetry (DSC), X-ray diffractometry, light microscopy and at the granular level by transmission electron microscopy (TEM).
Coatings microstructures have been analysed by scanning- and transmission-electron microscopy and at varying substrate dilutions, have been found to comprise a 100 to 500 nm interdendritic austenitic phase and a dendritic dual-phase of ferrite/martensite.
Study Design: A case-control study of 62 otherwise healthy women who were attending a vaginitis clinic ≥3 times a year for symptoms of Candida vaginitis, positive microscopy, and at least one positive Candida culture and of 32 Candida -negative control subjects, all of whom were undergoing standardized oral glucose tolerance testing.
The photothermal and photodynamic cytotoxicity of these mTHPC/MWCNT on/off complexes was assessed at the cell level by viability test, imaging flow cytometry, confocal microscopy and transmission electron microscopy and at the molecular level by a proteomic analysis of apoptosis-related proteins and genomic analysis of 84 genes involved in oxidative stress.
The GFP signal was then examined by fluorescence microscopy and at least 200 individual asci were scored as to whether GFP was seen only in the spore wall or throughout the ascal cytoplasm.
For numerical counts of Snail1 subcellular localization, images were acquired with Leica confocal microscopy and at least 400 cells in randomly selected high-power field (400×) were counted for each experiment.
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We thank the staff at the Heron Island Research Station for the use of the facilities, Dr. Kathryn Green at the Centre for Microscopy and Microanalysis at the University of Queensland for preparing EM sections, and Degnan lab members for constructive discussions.
Cells were confirmed to be lysed on light microscopy and centrifuged at 750 g for 10 min at 4°C.
Transmission electron microscopy (TEM) was taken with a JEOL model JEM2100 (JEOL Ltd., Tokyo, Japan) transmission electron microscopy and operating at 200 kV.
All technicians who read the slides had undergone appropriate training in malaria-related microscopy and had at least 5 years experience in reading blood smears.
Phage assays cannot replace conventional diagnostic tests such as microscopy and culture at this time.
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