SAS performed Laurdan GP microscopy and analysed the data obtained.
The slides were viewed using epifluorescent microscopy and analysed using NIS Elements (Nikon).
For basal ΔΨm measurements, Z-stack images were obtained by confocal microscopy and analysed using Zeiss software (Zeiss).
Functional vessel density, diameter of microvessels and red blood cell velocity were visualised by both orthogonal polarisation spectral™ imaging and fluorescence microscopy and analysed using a digital image system.
To study the effect of VANGL2 modulation on canonical WNT/ β-catenin signaling, we determined β-catenin levels by immunofluorescence microscopy and analysed β-catenin/TCF-dependent transcription in luciferase reporter assays before and after VANGL2 overexpression in SW480.
JP further carried out data acquisition, phylogenetic analyses as well as microscopy and Western analyses.
Measurements of basal and caerulein-induced amylase secretion, phase-contrast microscopy and immunohistochemical analyses were used to characterize the cultures.
Alamar Blue assay, scanning electron microscopy, confocal laser scanning microscopy and histological analyses were performed to assess hMSC adhesion, proliferation and 3-D colonization.
Scanning electron microscopy and thermogravimetric analyses showed that the loss of activity was due to carbon deposition.
The granules were resuspended in 100 µl of HEPES buffer and were used for the various microscopy and spectroscopy analyses.
