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Light microscopy analyzes were performed at the University of Padova, Italy.
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For electron microscopy analyzes eyes were harvested from Abca4−/− or sh1 mice at 3 and 2 3 months after AAV injection, respectively.
Optical microscopy, transmission electronic microscopy and scanning electron microscopy were adopted to analyze its morphological features according to the conventional methods (Chao et al. 2010; Deng et al. 2014, 2016; Prior and Perkins 1974).
The effects of the various drugs on the morphology of the interaction process, as evaluated using high-resolution scanning electron microscopy and transmission electron microscopy, were also analyzed.
Images from at least three different microscopy fields were analyzed per replicate.
For confocal microscopy, samples were analyzed using a Leica TCS SP2 confocal microscope.
Then electron microscopy images were analyzed using ImageJ software.
To evaluate the SR-BI localization at the apical and basolateral domains of the plasma membrane, confocal microscopy images were analyzed in 3D with Imaris software v7.7.2 (Bitplane, South Windsor, CT).
Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
Scanning electron microscopy and atomic force microscopy were used to analyze surface morphology of the wires which were then immersed in artificial saliva for 1 month to determine potential nickel ions' release by means of atomic absorption spectrophotometer.
Assisted by cryogenic electron microscopy, these nanoparticles were analyzed in detail and found to present vastly diverse shapes and highly ordered inner mesostructures.
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