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For electron microscopy analysis, loaded beads were resuspended in the fixative solution.
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Confocal microscopy analysis showed that similar to our in vitro data, tumor cells recovered from tumors injected with lipo-ODN were loaded with substantial quantities of both surface and internalized lipo-ODN.
A SEM (scanning electron microscopy) analysis was carried out in order to evaluate the microstructural modifications due to the environments and cyclic loads.
Microscopy analysis was conducted as previously described [38].
Confocal microscopy analysis was performed using a Zeiss LAM 510Meta.
Thin sections were then routinely prepared for light microscopy analysis.
Thanks to Tatjana Iezhova for help with the microscopy analysis.
We thank Annarita Taddei for the electron microscopy analysis.
EWK did microscopy analysis.
C.J.S. performed optical microscopy analysis.
CJ participated in microscopy analysis.
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