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Considerations for setting up microscopy analysis included the fixing protocol, choice of growth medium, viable cell counting, cross-talk between fluorophores, as well as the method of 3D visualization.
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Microscopy analysis including Scanning Electron Microscope (SEM) Analysis, X-Ray Diffraction (XRD) Analysis and X-Ray Fluorescence (XRF) Analysis are conducted to understand the macroscopic failure phenomenon, element composition and concrete hydration process.
Analysis included macroscopy, light microscopy with routine hematoxylin and eosin staining (cellularity and tissue structure), and immunohistochemical studies to allow identification of macrophages (CD68) and T lymphocytes (CD3), endothelial cells, leukocyte adhesion molecules (CD54, CD106, and CD62E), and immunoglobulin (IgG) and complement factor (C3) depositions.
CSF analysis included biochemistry, cytology, microbiology (including microscopy and culture for fungi and pyogenic bacteria), syphilis serology, HIV load, and Cryptococcus latex agglutination titer.
The analysis included participants who were AFB smear-negative on both ZN and FM microscopy and had TB blood culture results available.
beta1 integrin analysis included alpha1beta1 (CD49alpha2beta1etalpha3beta1 alpha4beta1 (CD49c), alpha5beta1 (CD49d), andhalpha6beta149e), and alpha6beta1 (CD49f) receptors, and was carried out by reverse transcriptase-polymerase chain reaction, confocal microscopy and flow cytometry.
Data analysis included frequency reporting.
Urine analysis (including microscopy) was performed in second spot urine.
Laboratory findings: Clinical chemistry, hematology, lipids, coagulation factors, hormones and urine analysis (including microscopy) were analyzed in all subjects who completed the full dosage regimen (n = 64).
ICU patients from whom stool samples had been sent for microbiological analysis (including microscopy and C. difficile toxin (CDT)) were assumed to have suffered diarrhoea.
Detailed micro- and meso-structural analysis including tomography, microscopy and EBSD analysis revealed substantial local material property variations within the lattice structure.
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