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The allergen-copolymer nanoaggregate was characterized by means of DLS, zeta potential, electrophoretic mobility and atom force microscopy analysis displaying the formation of a stable complex.
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Fluorescence microscopy analysis showed that the bipD mutant displayed a high level of co-localization with LC3 at 2 h, 4 h and 6 h p.i. (Figure 5C).
Fluorescence microscopy analysis revealed that human IgG fractions displayed a stronger binding to the surface of HUVECs undergoing apoptosis as compared with untreated cells.
Scanning electron microscopy analysis (see Fig. 1b) revealed that La0.6Sr0.4Co0.2Fe0.8O3 particles displayed an irregular morphology and were agglomerated with each other.
A transmission electron microscopy analysis confirmed the alteration of mitochondria in senescent cells which displayed very dense and thickened cristae (Figure 2B).
Indeed, transmission electron microscopy analysis showed EspA filaments with an average length of 700±150 nm displayed on EPEC bacteria, which were detected by anti-EspA and immunogold.
Electron microscopy analysis of co-cultures of BMHC and MDA-MB231 or MCF7 displayed close interactions with formation of tight junctions (Fig. 1b).
Microscopy analysis was conducted as previously described [38].
Confocal microscopy analysis was performed using a Zeiss LAM 510Meta.
Thin sections were then routinely prepared for light microscopy analysis.
Thanks to Tatjana Iezhova for help with the microscopy analysis.
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