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For electron microscopy affinity purified antibodies against the C-terminal (CT) part of PDE3B were generated in rabbit using the peptide NASLPQADEIQVIEEA.
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Although some degree of NP adsorption on the biomass was observed using fluorescence microscopy, the affinity of SiO2 NPs for the activated sludge was not enough for a sustained and effective removal of the SiO2 NPs from the wastewater.
Immunofluorescence microscopy with affinity purified CNGC-specific antisera indicated that AtCNGC5 and AtCNGC10 are present in the plasma membrane of protoplasts.
To determine whether these LDCVs express the secretory granule marker proteins, the expression of the two major granin proteins chromogranin B (Fig. 2A) and secretogranin II (Fig. 2B) was investigated by immunogold electron microscopy using affinity-purified CGB and SgII antibodies.
To verify the asymmetric distribution, we determined the localization of native RomR in the wild type by immunofluorescence microscopy using affinity-purified RomR antibodies.
Therefore, RGO can be re-dispersed in water and 2 3 layers of graphene can be observed by transmission electron microscopy, showing excellent affinity with water.
All Anticalins can be readily expressed in Escherichia coli as soluble and strictly monomeric proteins, and they show specific staining of ED-B-positive tumor cells in immunofluorescence microscopy while BIAcore affinity analyses indicate recognition of distinct ED-B epitopes.
Among the diseases of protein misfolding, amyotrophic lateral sclerosis (ALS) is unusual in that the proteinaceous neuronal inclusions that are the hallmark of the disease have neither the classic fibrillar appearance of amyloid by transmission electron microscopy nor the affinity for the dye Congo red that is a defining feature of amyloid.
We have developed a semi-automated technique called spatially targeted optical microproteomics (STOMP), which combines two-photon laser scanning microscopy with photochemical affinity labeling and mass spectrometry.
With the estimation of klat from Equation 1, the lateral force (Flat) can be calculated from the measurement of the lateral force signal in LFM analysis such as [36] F lat = k lat × S lat × Δ V (2) Figure 5 Lateral force microscopy analysis of binding affinity between AuNPs and chemically functionalized surfaces.
We used the confocal microscopy to evaluate the affinity of TCTPs to the receptor (Figure 6).
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