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Periodically, organoids were removed, under microscopic visualization, for propagation into new culture flasks or phenotypic and molecular analysis.
Research has relied on animal models of infection as a source of organisms for biochemical testing, drug evaluation, and microscopic visualization for life cycle analyses.
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For microscopic visualization of colonization in fish embryo, 5-day old fish embryos were infected with E. coli Top10 derivatives strains harboring a green fluorescent protein expression plasmid alongside desired recombinant constructs cloned in vectors with replicon compatibility.
Imaging capabilities made possible with the β-lactamase reporter system allow for microscopic visualization of reporter enzyme activity due to cleavage of the FRET-based substrate.
This technique allows for microscopic visualization of any microorganisms that reside on the surface of the skin or within the ear canal cerumen.
Longitudinal 6 μm thick sections were performed with a Leica SM 2500s microtome and were stained using a combination of Safranin-O/von Kossa for microscopic visualization of mineralized bone matter.
Sections dedicated for fluorescent microscopic visualization of DiI-labeled cells were not stained with toluidine blue, and nuclei were counterstained by 4',6-diamidino-2-phenylindole dihydrochloride.
We agree with those authors that direct diagnosis by microscopic visualization of amastigotes is the first choice for the diagnosis of leishmaniasis.
Other standard techniques for IC diagnosis including microscopic visualization of the infecting fungus and histopathology, usually lack specificity or sensitivity or require invasive procedures that cannot be performed because of the patient's condition [ 5].
Recently, noninvasive imaging has been investigated for the in vivo near microscopic visualization of the vaginal epithelium in small and large animal models.
The current diagnostic "gold standard" for malaria diagnosis is direct microscopic visualization of parasites on thick and/or thin blood smears [ 4, 5].
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