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This approach has been performed thanks to the use of self-fluorescent microorganisms (modified microorganisms) and the design of a specific filtration cell allowing a direct microscopic visualisation of the deposit to be performed without any treatment which could damage or modify the structure.
A representative confocal microscopic visualisation of interaction between C. jejuni (green) and human small intestine tissue (red) is shown in Figure 1. C. jejuni were routinely found in close association with the epithelial lining (Figure 1a), with particular propensity for micro-colony formation while adhering to the small intestine (Figure 1b).
(D) Confocal microscopic visualisation of VIM expression in T47D cells with either forced or depleted expression of TFF3.
(A) Confocal microscopic visualisation of f-actin arrangement in T47D cells with either forced or depleted expression of TFF3.
Isolation of organoid preparations was consistent between control and experimental groups, and although organoid sizes vary, this variability appeared consistent by microscopic visualisation of the organoids before injection.
(C) Confocal microscopic visualisation of CDH1 expression in MCF7 and T47D cells with forced expression of TFF3 after exposure to JSI-124 (0.2 μM) or Stattic (2 μM).
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Selection and isolation of the candidate cells and/or microscopic areas to be captured was performed under direct microscopic visualisation, by using the Pix Cell II Laser Capture Microscope (Arcturus Engineering, Mountain View, CA, USA).
All invading cells were counted by microscopic visualisation.
Pgp expression (see Immunofluorescence staining for Pgp expression) and the intracellular localisation of MTZ and hypericin were investigated by growing cells on glass for microscopic visualisation.
Cell populations were estimated to be homogeneous as determined by microscopic visualisation.
Once the cervico-occipital dura is well exposed, a vertical incision of the outer layer will be performed, and the outer layer of the dura will be separated and removed from the inner layer caudally and laterally under microscopic visualisation.
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