CD68+ only or CD68+/ E. coli + cells were isolated under direct microscopic visualisation (Fig. 1d, e) and catapulted onto a PALM adhesive cap.
Once the cervico-occipital dura is well exposed, a vertical incision of the outer layer will be performed, and the outer layer of the dura will be separated and removed from the inner layer caudally and laterally under microscopic visualisation.
(C) Confocal microscopic visualisation of CDH1 expression in MCF7 and T47D cells with forced expression of TFF3 after exposure to JSI-124 (0.2 μM) or Stattic (2 μM).
A representative confocal microscopic visualisation of interaction between C. jejuni (green) and human small intestine tissue (red) is shown in Figure 1. C. jejuni were routinely found in close association with the epithelial lining (Figure 1a), with particular propensity for micro-colony formation while adhering to the small intestine (Figure 1b).
All patients had a standard operative approach to the cervical spine, through a left sided collar incision, with the operating surgeon using Loupes and headlight initially, with microscopic visualisation at the cervical disc level.
Selection and isolation of the candidate cells and/or microscopic areas to be captured was performed under direct microscopic visualisation, by using the Pix Cell II Laser Capture Microscope (Arcturus Engineering, Mountain View, CA, USA).
Isolation of organoid preparations was consistent between control and experimental groups, and although organoid sizes vary, this variability appeared consistent by microscopic visualisation of the organoids before injection.
Pgp expression (see Immunofluorescence staining for Pgp expression) and the intracellular localisation of MTZ and hypericin were investigated by growing cells on glass for microscopic visualisation.
This approach has been performed thanks to the use of self-fluorescent microorganisms (modified microorganisms) and the design of a specific filtration cell allowing a direct microscopic visualisation of the deposit to be performed without any treatment which could damage or modify the structure.
All invading cells were counted by microscopic visualisation.
