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We characterized the immunoreactivity of aquaporin-1 and aquaporin-4 (and1 AQP4and4) associatedated neuropathology in microscopic transverse sections from archival autopsied pontine tissue from 6 patients with pathologically confirmed CPM.
Digital images of 7 randomly selected microscopic transverse sections per treated animal were analyzed and the percentage of immunostained A β deposition was determined for all the markers studied, as defined by the stereotaxic Bregma coordinates.
The quantification of A β plaque was determined in randomly selected microscopic transverse sections per animal group from a total of 7 sections per animal/group and were evaluated using the HIH Image J program by defining region of interest and setting a threshold to discriminate nonspecific staining.
To characterize AQP1 and AQP4 immunoreactivity and neuropathological characteristics of CPM, we analyzed microscopic transverse sections of the pons in archival autopsied tissue from two control cases (17 year old female and 15 year old male), and six pathologically confirmed CPM cases.
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Scale bars = 1 μm. Figure 2 Microscopic observations of transverse sections of fully developed leaf blades of seedlings.
Furthermore, microscopic observations of leaf transverse sections indicated that hyperaccumulation of starch in chloroplasts of mesophyll and bundle sheath cells occurred in lse2 and lse3 plants, while that in vascular cells was noticeable only in lse3 leaves.
The existence of isolated tumor deposits in the mesorectum was well demonstrated by microscopic examination with serial transverse sections of the mesorectum made as reported by Scott et al [ 16] and Reynolds et al [ 17].
Microscopic examination of the unstained transverse sections under bright field showed that not only are the AVI forms in the adaxial epidermis different between the outer and inner petal regions, but also the AVI forms differ more greatly in the adaxial epidermal cells than in the abaxial epidermal cells of the same inner petal region (Fig. 1A).
Lung transverse sections (thickness, 4 μm) were sliced and mounted on microscopic slides.
Images are transverse sections of cichlid embryos.
Transverse sections were stained with hematoxylin and eosin.
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