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Curating and monitoring these will require non-invasive microscopic technologies of the kind presented here.
Further progress will be fuelled by emerging novel approaches and methodologies such as in vivo ligand detection and determination and single cell biophysical and microscopic technologies to detect ligand receptor and ligand co-factor interactions in vivo.
Although bioimage-informatics has a rather long history and thus it is easy to find many past trials especially on blood cell segmentation and cell counting (such as Parthenis et al. 1989), recent progress in microscopic technologies is enhancing the research activity.
We can rely on history to repeat itself in that further studies using these cutting-edge microscopic technologies will change our perception of Leishmania spp. and T. cruzi intracellular parasitism and contribute to the development of novel and more efficient strategies of chemotherapy and vaccination.
Due to the complex tubular structure and other factors such as large size, various micro- and nanotopographies, and translucent or opaque materials, it is almost impossible to investigate the cell pore interactions within the scaffold during culture using currently available microscopic technologies.
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Achievements of live cell imaging and efficient spatiotemporal resolution however have been possible only with the advent of fluorescence microscopic technology, equipped with precisely sensitive automated softwares.
Advances in microscopic technology have revolutionized the way objects can be perceived on the nanoscale.
In present study, the development of sporangium and spore of H. serrata was studied using microscopic technology.
Therefore, developing a microscopic technology with noninvasive, in situ, label-free, single-cell spatial resolution may serve this long-term need.
Thirdly, we used a new microscopic technology SICM [28] to demonstrate the morphological and topographical features of various cell structures during culture with high resolution (<0.1 μm) [28].
In recent years, confocal microscopic technology in conjunction with a new generation of fluorescent dyes has been widely used for dynamic changes analysis of calcium ions in living cells.
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