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Electron microscopic techniques such as cryo-electron tomography are remarkable tools for imaging the interiors of bacterial cells in the near-native state, whereas optical microscopic techniques such as fluorescence imaging are useful for following the dynamics of specific single molecules in living cells.
Although this technique is macroscopic, contrary to other macroscopic methods such as thermogravimetrical and chromatographic techniques, the diffusivities obtained are in agreement with those determined using microscopic techniques such as NMR.
These identifications were accompanied by diverse microscopic techniques such as fluorescence microscopy and conventional scanning electron microscopy.
These key parameters are usually determined after synthesis of nanostructures using electron microscopic techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
This reflects the scale of the murine vasculature – too large for microscopic techniques such as single-photon confocal microscopy but too small for techniques derived from the clinic, including magnetic resonance imaging (MRI [3] and computed tomography (CT [4].
Therefore, microscopic techniques such as microhematocrit and concentration methods in umbilical cord blood have fairly high (>80%) sensitivity (14 ).
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With the advances in pulsed laser systems, microscopic imaging techniques such as multiphoton and pump-probe fluorescence microscopy have developed into effective tools for investigating intensity and time-resolved phenomena inside biological systems.
However, within the past two decades, Charcot's initial descriptions enjoyed a revival, mainly by the advent of advanced microscopic imaging techniques, such as the combination of fluorescent immunocytochemistry with confocal microscopy.
The recent developments and future perspectives of contemporary microscopic imaging techniques such as three-dimensional and in vivo imaging are analyzed for their clinical potentials.
In order to characterize BA at different microscopic levels, a combination of techniques such as Solid State Nuclear Magnetic Resonance (ssNMR), Scanning Electron Microscope (SEM), micro-computed tomography (μCT) and Thermal Gravimetric Analysis (TGA) were used for delineating the ultra-structural property of bone.
Developments of other new tools or techniques, such as microscopic NIEL degradation prediction tools, will be outlined.
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