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With electron microscopic studies using the tracer HRP (horse radish peroxidase) we demonstrated that the PPE is associated with increased transcytotic transport.
Pore incorporation was investigated via macroscopic electrochemical characterization using cyclic voltammetry (CV), and microscopic studies using atomic force microscopy (AFM) and AFM-scanning electrochemical microscopy (SECM) recording current-distance curves at the supported lipid membranes (SLMs) before and after the incorporation of pores.
Microscopic studies using these antibodies for double and triple labeling demonstrate sequential phosphorylation and dephosphorylation during the cell cycle.
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However, the majority of these electron microscopic studies used permanganate fixation, which would destroy the MFIBs.
To analyse the localization of filamin in dividing GAPA− cells we perfomed microscopic study using GAPA− GFP-FLN cells treated with nocodazole.
From extensive studies using microscopic approaches, and more recently molecular and genomic methods, a detailed view of the 3D arrangement of chromosomes inside interphase nuclei is emerging [ 1– 8, 38, 41].
Early studies using electron microscopic analysis reported remodeling of gill tissues and identified changes in the relative abundance of PVCs and MRCs in fish acclimated to waters of different salinities [ 2, 3].
Efficient and active internalization of mesoporous silica particles of different sizes was observed by transmission electron microscopic and flow cytometric analysis and studies using pharmacological inhibitors suggested that uptake occurred through a process of endocytosis.
HT-29LMM cells were used for further experiments due to better comparability with results from earlier studies using a similar microscopic technique within the liver.
Quantifying these details, especially in micrometer and nanometer scale structures, requires extensive experimental studies using a suite of microscopic and spectroscopic techniques (see, for example,[82, 87, 89]).
The atomistic information derived from these microscopic studies was used to discuss the thermodynamic data and to verify the theoretical models of the interface.
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