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Macroscopic and microscopic specimens were evaluated by a pathologist in a blind fashion.
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In year 3 students attend laboratory courses in which microscopic and macroscopic specimens are viewed.
For light microscopic observation, ileum specimens were embedded in the paraffin blocks after they had been fixed in Bouin's solution.
For light microscopic studies, duodenum specimens were fixed in Bouin fluid for 48 h, dehydrated in an ascending series of ethyl alcohol, and embedded in paraffin.
Prior to scanning electron microscopic observations, the HAM specimens were dehydrated in a graded ethanol series of 25, 50, 75, 95 and 100%% each for 10 min.
For microscopic evaluation, colonic tissue specimens were snap-frozen in liquid nitrogen and stained with hematoxylin-eosin (HE).
Microscopic slides from patient specimens were retrieved from an archive and reanalyzed.
For light microscopic examination, renal biopsy specimens were fixed in 10% phosphate-buffered formalin (pH 7.2), embedded in paraffin, and sliced into sections 4 μm thick.
For immunofluorescence histochemistry and pre-embedding electron-microscopic immunocytochemistry, the fixed specimens were rinsed overnight in the same phosphate buffer containing 30%% sucrose at 4 °C and then rapidly frozen in isopentane precooled to −35 °C.
Microscopic examination and photography of specimens were performed with a Zeiss Axiovert 200 direct epifluorescence microscope.
Specimens were transferred onto microscopic slides and examined under a compound microscope.
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