The obtained formulations are first applied to crystallographic materials, and a crystal gradient plasticity model is developed to account for the influence of microscopic slip rearrangements on the macroscopic gradient-dependent mechanical behaviour for this class of materials.
Following induction, cells are placed on a microscopic slide between a cover slip and 0.8% LB-agarose gel pad set, and visualized by fluorescence microscopy, using a Nikon Eclipse TE2000-U, TE2000-U,okyo, JapaNikonverTokyo, Japancal laser-scanninvertedem with a 100× Apo TIRF (1.49 NA, oil) objeC1ive.
In order to apply an external stress on the leaf epidermis, we placed leaves from Arabidopsis plants expressing the microtubule marker TUA6-GFP between a microscopic slide and a cover slip and observed that prolonged incubation induces a strong alignment of microtubules within adaxial epidermal pavement cells.
The percentage of Neu-N or SOX-2 positive cells was calculated from two different cover-slips in a total of eight independent microscopic fields (4 microscopic fields per cover-slip) and normalized to total number of nuclei with DAPI staining (approximately 70 nuclei per microscopic field).
The suspension was spread on a frosted microscopic slide pre-coated with 250 μL of 0.8% normal melting agarose, covered with a cover slip, and then allowed to solidify on ice for 10 min.
It is not possible to judge the relative importance of slip and twinning in the deformation of HMX, under the conditions encountered during indentation from purely microscopic observations around the impressions.
Cultured cells: VLPs were added to subconfluent cells, in a small volume of serum-free medium, grown in 25 cm2 tissue-culture bottles (for total protein extraction and Western analyses) or on microscopic cover slips (for phase and confocal microscopy).
Slip and slide, slip and slide.
One slip and they'd be out.
One slip and the apple is gone.
