Sentence examples for microscopic slides were from inspiring English sources
In brief, frosted microscopic slides were covered with 0.5% normal melting agarose at 60°C.
Microscopic slides were prepared by spreading 1 ml of 1% molten agarose +0.5 µg/ml of the lipid probe Nile Red.
New haematoxylin-eosin stained microscopic slides were prepared from the chosen blocks and again checked for their technical as well as for their diagnostic adequacy.
Pollen pellets were separated according to the color and microscopic slides were prepared by mounting unacetolyzed pollen in high viscosity silicone oil [Poly (dimethylsiloxane), 200® fluid, viscosity 30,000 cSt].
Cells seeded on microscopic slides were incubated in DMEM 5% FCS for 2 hours in the presence of excess of irradiated (600 rad) thymocytes, previously labelled for 20 min at RT with 7-AAD (20 µg/ml in PBS – Sigma Aldrich, St .Louis, Missouri, USA).
Subsequently cells were exposed to an alkaline buffer (300 mM NaOH, 1 mM disodium EDTA) for 30 min. The microscopic slides were subjected to electrophoresis at 0.8 V/cm−1 for 30 min after which slides were immersed in a neutralization buffer (0.4 M Tris, pH 7.5) for 15 min. After staining with ethidium bromide (20 µg/ml) slides were analyzed under a fluorescence microscope (Nikon PCM-2000).
End frosted microscopic slides are used for the SCGE assay.
First, intercept lengths measured on microscopic slides are primarily determined by the inflation level at which the specimen has been held during tissue preparation.
Quantitative evaluation on the microscopic slides was performed by measuring with a scale and with imaging analysis software (NIS-Elements D 3.00, Nikon, Tokyo, Japan).
However, while the routine pathologist who reevaluated the microscopic slides was blinded towards clinical and macroscopic data, the senior pathologist not only reevaluated the microscopic slides but also considered information from the operative and macroscopic reports.
Chromosomes sorted onto microscopic slides are an attractive alternative as they are completely free of cytoplasmic contamination and facilitate high-resolution analysis on large populations of chromosomes (Lucretti et al. 1993).
